https://cdn.earthdata.nasa.gov/conduit/upload/5182/KeywordsCommunityGuide_Baseline_v1_SIGNED_FINAL.pdf 23.9 2026-05-13T10:52:13.516Z https://gcmd.earthdata.nasa.gov/KeywordViewer/scheme/instruments/35dc5fc4-4c96-44d9-8710-0e99e00201c8 FLUORESCENCE MICROSCOPY Date=2021-08-05 User Id=sritz Entity=AltLabel Operation=INSERT System Note=insert AltLabel New Value=id: null category: primary text: FLUORESCENCE MICROSCOPY language code: en Fluorescence illumination and observation is the most rapidlyexpanding microscopy technique employed today, both in themedical and biological sciences, a fact which has spurred thedevelopment of more sophisticated microscopes and numerousfluorescence accessories. Epi-fluorescence, or incident lightfluorescence, has now become the method of choice in manyapplications and comprises a large part of this tutorial. Wehave divided the fluorescence section of the primer into severalcategories to make it easier to organize and download. Pleasefollow the links below to navigate to points of interest.Introductory Concepts - Fluorescence is a member of theubiquitous luminescence family of processes in which susceptiblemolecules emit light from electronically excited states createdby either a physical (for example, absorption of light),mechanical (friction), or chemical mechanism. Generation ofluminescence through excitation of a molecule by ultraviolet orvisible light photons is a phenomenon termed photoluminescence,which is formally divided into two categories, fluorescence andphosphorescence, depending upon the electronic configuration ofthe excited state and the emission pathway. Fluorescence is theproperty of some atoms and molecules to absorb light at aparticular wavelength and to subsequently emit light of longerwavelength after a brief interval, termed the fluorescencelifetime. The process of phosphorescence occurs in a mannersimilar to fluorescence, but with a much longer excited statelifetime.Anatomy of the Fluorescence Microscope - In contrast to othermodes of optical microscopy that are based on macroscopicspecimen features, such as phase gradients, light absorption,and birefringence, fluorescence microscopy is capable of imagingthe distribution of a single molecular species based solely onthe properties of fluorescence emission. Thus, usingfluorescence microscopy, the precise location of intracellularcomponents labeled with specific fluorophores can be monitored,as well as their associated diffusion coefficients, transportcharacteristics, and interactions with other biomolecules. Inaddition, the dramatic response in fluorescence to localizedenvironmental variables enables the investigation of pH,viscosity, refractive index, ionic concentrations, membranepotential, and solvent polarity in living cells and tissues.Additional information available at:'http://micro.magnet.fsu.edu/primer/techniques/fluorescence/fluorhome.html' 2021-08-05 11:53:31.0